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primary human umbilical artery smcs (huasmcs)  (Lonza)


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    Lonza primary human umbilical artery smcs (huasmcs)
    Primary Human Umbilical Artery Smcs (Huasmcs), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+umbilical+artery+smcs+(huasmcs)/human+umbilical+artery+smooth+muscle+cells/pm26987444-27-0-7
    Average 90 stars, based on 1 article reviews
    primary human umbilical artery smcs (huasmcs) - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Isolation, differentiation and characterization of vascular cells derived from human embryonic stem cells
    Article Snippet: hESCs cell lines H9 and H13 (Wicell International Stem Cell Bank, Wicell Research Institute) Human umbilical vein endothelial cells (HUVECs) (Clonetics, Lonza, cat. no. C2517A) Human umbilical artery SMCs (HUASMCs) (Clonetics, Lonza, cat. no. CC-2579) MEF (see REAGENT SETUP) Growth media and supplements DMEM (Invitrogen, cat. no. 41965-035) Knockout DMEM (Invitrogen, cat. no. 10829-018) DMEM–F12 (Biological Industries, cat. no. 01-170-1A) Knockout serum replacement (KSR) (Invitrogen, cat. no. 10828-028) Fetal bovine serum (FBS) (Hyclone, Thermo Fisher Scientific, cat. no. SH30070.03) L-glutamine (200 mM in 0.85% NaCl) (Invitrogen, cat. no. 25030-081) β-Mercaptoethanol (55 mM in PBSA) (Invitrogen, cat. no. 21985-023) ! cautIon β-Mercaptoethanol is toxic.

    Article Title: Arterial graft with elastic layer structure grown from cells.
    Article Snippet: Human umbilical artery SMCs (hUASMCs) were obtained from Lonza (Walkersville, MD).

    Article Title: Isolation, differentiation and characterization of vascular cells derived from human embryonic stem cells
    Article Snippet: REAGENTS Cells list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 hESCs cell lines H9 and H13 (Wicell International Stem Cell Bank, Wicell Research Institute) Human umbilical vein endothelial cells (HUVECs) (Clonetics, Lonza, cat. no. C2517A) Human umbilical artery SMCs (HUASMCs) (Clonetics, Lonza, cat. no. CC-2579) MEF (see REAGENT SETUP) Growth media and supplements list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 DMEM (Invitrogen, cat. no. 41965-035) Knockout DMEM (Invitrogen, cat. no. 10829-018) DMEM–F12 (Biological Industries, cat. no. 01-170-1A) Knockout serum replacement (KSR) (Invitrogen, cat. no. 10828-028) Fetal bovine serum (FBS) (Hyclone, Thermo Fisher Scientific, cat. no. SH30070.03) L-glutamine (200 mM in 0.85% NaCl) (Invitrogen, cat. no. 25030-081) β-Mercaptoethanol (55 mM in PBSA) (Invitrogen, cat. no. 21985-023) !

    Article Title: Isolation, differentiation and characterization of vascular cells derived from human embryonic stem cells
    Article Snippet: hESCs cell lines H9 and H13 (Wicell International Stem Cell Bank, Wicell Research Institute) Human umbilical vein endothelial cells (HUVECs) (Clonetics, Lonza, cat. no. C2517A) Human umbilical artery SMCs (HUASMCs) (Clonetics, Lonza, cat. no. CC-2579) MEF (see REAGENT SETUP)

    Article Title: Unraveling the role of mechanical stimulation on smooth muscle cells: A comparative study between 2D and 3D models.
    Article Snippet: Athoroughunderstanding of cell response to combined culture configuration and mechanical cues is of paramount importance in vascular tissue engineering applications.. Herein, we investigated and compared the response of vascular smooth muscle cells (vSMCs) cultured in different culture environments (2D cell monolayers and 3D cellularized collagen-based gels) in combination with mechanical stimulation (7% uniaxial cyclic strain, 1 Hz) for 2 and 5 days.When cyclic strainwas applied, two different responses, in terms of cell orientation and expression of contractile-phenotype proteins, were observed in 2D and 3D models.. Specifically, in 2D configuration, cyclic strain caused 50% of cell population to align nearly perpendicular (80–90 degrees) to the strain direction, while not influencing the contractile-phenotype protein expression, as compared to the 2D static controls.

    Article Title: Isolation, differentiation and characterization of vascular cells derived from human embryonic stem cells
    Article Snippet: Cells list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 hESCs cell lines H9 and H13 (Wicell International Stem Cell Bank, Wicell Research Institute) Human umbilical vein endothelial cells (HUVECs) (Clonetics, Lonza, cat. no. C2517A) Human umbilical artery SMCs (HUASMCs) (Clonetics, Lonza, cat. no. CC-2579) MEF (see REAGENT SETUP) Growth media and supplements list-behavior=simpleprefix-word=mark-type=nonemax-label-size=0 DMEM (Invitrogen, cat. no. 41965-035) Knockout DMEM (Invitrogen, cat. no. 10829-018) DMEM–F12 (Biological Industries, cat. no. 01-170-1A) Knockout serum replacement (KSR) (Invitrogen, cat. no. 10828-028) Fetal bovine serum (FBS) (Hyclone, Thermo Fisher Scientific, cat. no. SH30070.03) L-glutamine (200 mM in 0.85% NaCl) (Invitrogen, cat. no. 25030-081) β-Mercaptoethanol (55 mM in PBSA) (Invitrogen, cat. no. 21985-023) !

    Modification:

    Article Title: A Dual-Mode Bioreactor System for Tissue Engineered Vascular Models.
    Article Snippet: In the past decades, vascular tissue engineering has made great strides towards bringing engineered vascular tissues to the clinics and, in parallel, obtaining in-lab tools for basic research.. Herein, we propose the design of a novel dualmode bioreactor, useful for the fabrication (construct mode) and in vitro stimulation (culture mode) of collagen-based tubular constructs.. Collagen-based gels laden with smooth muscle cells (SMCs) were molded directly within the bioreactor culture chamber.



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    Procell Inc human umbilical artery smcs huasmcs
    Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    Lonza human umbilical artery smcs (huasmcs) (clonetics, lonza, cat. no. cc-2579)
    Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    Lonza human umbilical artery smcs (huasmcs)
    Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    PromoCell human umbilical artery smcs huasmcs
    Effects of bare and coating-modified groups on <t>SMCs</t> proliferation. (A–C) Representative fluorescence images of <t>HUASMCs</t> cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
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    Image Search Results


    Effects of bare and coating-modified groups on SMCs proliferation. (A–C) Representative fluorescence images of HUASMCs cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Journal: Bioactive Materials

    Article Title: A hierarchical MgF 2 /polyurethane/pitavastatin coating alleviates degradation and enhances endothelialization of bioresorbable magnesium alloy stents

    doi: 10.1016/j.bioactmat.2025.08.038

    Figure Lengend Snippet: Effects of bare and coating-modified groups on SMCs proliferation. (A–C) Representative fluorescence images of HUASMCs cytoskeleton (red) and nucleus (blue) after 24 and 72 h of different treatments, including untreated control group, bare group, MgF 2 -coated group, MgF 2 /PU-coated group, and MgF 2 /PU/PTV-coated group (A), along with quantitative cell viability assessments at 24 h (B) and 72 h (C). (D–E) Relative mRNA expression levels of α-SMA (D), VCAM-1(E), as determined by qPCR analysis. One-way ANOVA followed by Tukey's multiple comparison test was performed to determine statistical significance. Data are presented as mean ± SD, with p -values <0.05 considered statistically significant (n = 3). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Article Snippet: Human umbilical vein ECs (HUVECs), human umbilical artery SMCs (HUASMCs), and MΦs were obtained from Procell Life Science & Technology Co., Ltd. All cell lines were maintained and passaged in a cell culture incubator at 37 °C with 5 % CO 2 .

    Techniques: Modification, Fluorescence, Control, Expressing, Comparison